Determination of epithelial tissue scattering coefficient using confocal microscopy


Collier T., Arifler D., Malpica A., Follen M., Richards-Kortum R.

IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS, cilt.9, sa.2, ss.307-313, 2003 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 9 Sayı: 2
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1109/jstqe.2003.814413
  • Dergi Adı: IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.307-313
  • Anahtar Kelimeler: cervix, confocal microscopy, epithelial tissue, scattering, OPTICAL-PROPERTIES, IN-VIVO, FLUORESCENCE SPECTROSCOPY, REFLECTANCE SPECTROSCOPY, DIFFUSE-REFLECTANCE, BREAST TISSUES, HUMAN SKIN, AUTOFLUORESCENCE, DYSPLASIA, DIAGNOSIS
  • Orta Doğu Teknik Üniversitesi Kuzey Kıbrıs Kampüsü Adresli: Hayır

Özet

Most models of light propagation through tissue assume the scattering properties of the various tissue layers are the same. The authors present evidence that the scattering coefficient of normal cervical epithelium is significantly lower than values previously reported for bulk epithelial tissue. They estimated the scattering coefficient of normal and precancerous cervical epithelium using measurements of the reflectance as a function of depth from confocal images. Reflectance measurements were taken from ex vivo cervical biopsies and fit to an exponential function based upon Beer's law attenuation. The mean scattering coefficients derived were 22 cm(-1) for normal tissue and 69 cm(-1) for precancerous tissue. These values are significantly lower than previously reported for bulk epithelial tissues and suggest that scattering of bulk tissue is dominated by the stroma. They also suggest that computational models to describe light propagation in epithelial tissue must incorporate different scattering coefficients for the epithelium and stroma. Further, the lower scattering of the epithelium suggests greater probing depths for fiber optic probes used by optical diagnostic devices which measure reflectance and fluorescence in epithelial tissue. The difference in scattering between normal and precancerous tissue is attributed to increased nuclear size, optical density, and chromatin texture. The scattering coefficient's measured here are consistent with predictions of numerical solutions to Maxwell's equations for epithelial cell scattering.